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Five unique GSTP1 -specific shRNA plasmids (clones TRCN0000083 773 , TRCN0000083 774 , TRCN0000083 775 , TRCN0000083 776 and TRCN0000083 777 ) and a negative control plasmid (empty vector control, SHC001) were purchased as glycerol stocks and plasmid DNA extracted using plasmid DNA maxi prep kits (Qiagen, Manchester, UK) according to the manufacturers instructions

products LONG R IGF-I Cell Culture Supplement 200 times more potent and two times more stable in cell culture than insulin, LONG R 3 IGF-I (LONG R 3 ) is a recombinant analog of human insulin-like growth factor-I (IGF-I) that has been specifically engineered for the enhancement of cell culture performance

Because of its high prevalence in conjunction with obesity, diabetes, and insulin resistance, NAFLD has been increasingly appreciated as a hepatic manifestation of metabolic syndrome and is the leading cause of death from liver disease in the United States 1,2,3

Labeled larvae were then rinsed once with PBS and stored in 1:1 PBS:glycerol for confocal imaging

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